Laboratory vacuum glove boxes adopt a dual-chamber sealing vacuum system to avoid direct exposure of the main working area to atmospheric air during material access. The whole system is equipped with oil-sealed vacuum pumps that quickly evacuate air from the transfer antechamber to achieve low-pressure vacuum status. All connecting flanges, glove ports and observation windows use silicone sealing rings to prevent air leakage, which is critical for long-term vacuum retention. The standard operation follows the three-evacuation-three-purging rule: pump the antechamber to vacuum, fill inert gas, and repeat three times to dilute residual air to ppb-level impuritiesUniversity…. The main chamber never undergoes full vacuum pumping; it maintains slight positive inert pressure while the antechamber undertakes all vacuum treatment tasks. Real-time pressure sensors automatically monitor vacuum values, and automatic valves adjust pumping and gas filling to stabilize the internal environment. This vacuum design effectively protects air-sensitive samples such as lithium metal and organic catalysts from oxidation and moisture erosion.


